Structured Summary
Abstract
A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography.
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Synonyms
5 entry terms
- Affinity Purification, Tandem
- Tandem Affinity Chromatography
- Affinity Chromatography, Tandem
- Chromatography, Tandem Affinity
- Tandem Affinity Chromatographies
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Aspects Covered
10 allowable subheadings
Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.
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History Note
2019
MeSH Record
Previous Indexing
- Chromatography, Affinity (2002-2018)
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AMA Style
References
- National Library of Medicine. Tandem Affinity Purification. Medical Subject Headings (MeSH). 2026. Unique ID D000078791. http://id.nlm.nih.gov/mesh/2026/D000078791
- Tandem Affinity Purification. In: Wikipedia. https://en.wikipedia.org/wiki/Tandem_affinity_purification
- Tandem Affinity Purification. In: Wikidata. https://www.wikidata.org/wiki/Q893051