Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Shotgun Sequencing

A laboratory technique for determining the DNA sequence of an organism's genome. In this approach, DNA of an organism is randomly broken into small DNA fragments which are then sequenced individually. Computational methods are then utilized to reassemble these sequenced fragments accurately, reconstructing the complete genome sequence.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

A laboratory technique for determining the DNA sequence of an organism's genome. In this approach, DNA of an organism is randomly broken into small DNA fragments which are then sequenced individually. Computational methods are then utilized to reassemble these sequenced fragments accurately, reconstructing the complete genome sequence.

MeSH Record

Classification

Broader headings

Related Concepts

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MeSH Record

Synonyms

5 entry terms
  • Whole Genome Shotgun
  • Whole Genome Shotgun Sequencing
  • Sequencing, Shotgun
  • Sequencings, Shotgun
  • Shotgun Sequencings

MeSH Record

Aspects Covered

13 allowable subheadings

Indexed with the subheadings adverse effects, classification, economics, ethics, history, instrumentation, mortality, nursing, psychology, standards, statistics & numerical data, trends, veterinary.

MeSH Record

History Note

2025

MeSH Record

Previous Indexing

  • Sequence Analysis, DNA (1983-2024)

MeSH Hierarchy

Tree Number

  1. E05.393.940w

AMA Style

References

  1. National Library of Medicine. Shotgun Sequencing. Medical Subject Headings (MeSH). 2026. Unique ID D000098625. http://id.nlm.nih.gov/mesh/2026/D000098625
  2. Shotgun Sequencing. In: Wikipedia. https://en.wikipedia.org/wiki/Shotgun_sequencing
  3. Shotgun Sequencing. In: Wikidata. https://www.wikidata.org/wiki/Q1073526