Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Second Harmonic Generation Microscopy

A microscopic imaging technique that takes advantage of the process of harmonic generation that occurs when photons interact to generate new photons of a different wavelength. In second harmonic generation, two photons of the same wavelength and frequency, such as from a LASER, interact inside a medium and are converted to a photon of twice the frequency and half of the wavelength of the two incident photons. The light signals captured are used to produce images that are dependent on the unique optical properties of the material.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

A microscopic imaging technique that takes advantage of the process of harmonic generation that occurs when photons interact to generate new photons of a different wavelength. In second harmonic generation, two photons of the same wavelength and frequency, such as from a LASER, interact inside a medium and are converted to a photon of twice the frequency and half of the wavelength of the two incident photons. The light signals captured are used to produce images that are dependent on the unique optical properties of the material.

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Synonyms

1 entry terms
  • Third Harmonic Generation Microscopy

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Aspects Covered

10 allowable subheadings

Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

MeSH Record

History Note

2018

MeSH Hierarchy

Tree Numbers

  1. E01w.370.350w.515w.717.500w
  2. E05.595w.717.500w

AMA Style

References

  1. National Library of Medicine. Second Harmonic Generation Microscopy. Medical Subject Headings (MeSH). 2026. Unique ID D000073798. http://id.nlm.nih.gov/mesh/2026/D000073798
  2. Second Harmonic Generation Microscopy. In: Wikipedia. https://en.wikipedia.org/wiki/Second-harmonic_imaging_microscopy
  3. Second Harmonic Generation Microscopy. In: Wikidata. https://www.wikidata.org/wiki/Q17092959