Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Nuclease Protection Assays

Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS.

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Broader headings

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MeSH Record

Synonyms

3 entry terms
  • Assay, Nuclease Protection
  • Assays, Nuclease Protection
  • Nuclease Protection Assay

MeSH Record

Aspects Covered

10 allowable subheadings

Indexed with the subheadings classification, economics, ethics, history, instrumentation, methods, standards, statistics & numerical data, trends, veterinary.

MeSH Record

History Note

2001

MeSH Hierarchy

Tree Number

  1. E05.393.600w

AMA Style

References

  1. National Library of Medicine. Nuclease Protection Assays. Medical Subject Headings (MeSH). 2026. Unique ID D023282. http://id.nlm.nih.gov/mesh/2026/D023282
  2. Nuclease Protection Assays. In: Wikipedia. https://en.wikipedia.org/wiki/Nuclease_protection_assay
  3. Nuclease Protection Assays. In: Wikidata. https://www.wikidata.org/wiki/Q6395811