Analytical, Diagnostic and Therapeutic Techniques, and Equipment

Chromosome Walking

A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.

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Synonyms

3 entry terms
  • Chromosome Walkings
  • Walking, Chromosome
  • Walkings, Chromosome

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Aspects Covered

2 allowable subheadings

Indexed with the subheadings instrumentation, methods.

MeSH Record

Indexing Annotation

technique for exploring an unknown region of a CHROMOSOME

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History Note

91

MeSH Record

Previous Indexing

  • Chromosome Mapping (1983-1990)
  • Cloning, Molecular (1983-1990)

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References

  1. National Library of Medicine. Chromosome Walking. Medical Subject Headings (MeSH). 2026. Unique ID D016386. http://id.nlm.nih.gov/mesh/2026/D016386
  2. Chromosome Walking. In: Wikipedia. https://en.wikipedia.org/wiki/Primer_walking
  3. Chromosome Walking. In: Wikidata. https://www.wikidata.org/wiki/Q3817120