Chemicals and Drugs

CRISPR-Associated Protein 9

An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS PYOGENES and other bacteria where it participates in an adaptive immune function to cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand, and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6 nucleotide DNA sequence immediately following the sequence targeted by Cas9.

National Library of MedicineMedical Subject Headings2026

Structured Summary

Abstract

An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS PYOGENES and other bacteria where it participates in an adaptive immune function to cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand, and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6 nucleotide DNA sequence immediately following the sequence targeted by Cas9.

MeSH Record

Classification

Broader headings

Related Concepts

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MeSH Record

See Also

MeSH Record

Synonyms

6 entry terms
  • Cas9 Endonuclease
  • Cas9 Enzyme
  • Cas9 Protein
  • CRISPR Associated Protein 9
  • Endonuclease, Cas9
  • Enzyme, Cas9

MeSH Record

Aspects Covered

29 allowable subheadings

Indexed with the subheadings administration & dosage, adverse effects, analysis, antagonists & inhibitors, biosynthesis, blood, cerebrospinal fluid, chemical synthesis, chemistry, classification, deficiency, drug effects, economics, genetics, history, immunology, isolation & purification, metabolism, pharmacokinetics, pharmacology, physiology, poisoning, radiation effects, standards, supply & distribution, therapeutic use, toxicity, ultrastructure, urine.

MeSH Record

History Note

2019

MeSH Record

Previous Indexing

  • Bacterial Proteins (2015-2018)
  • Endonucleases (2015-2018)

MeSH Hierarchy

Tree Numbers

  1. D08.811w.277w.352w.355w.325w.150w
  2. D12.776w.097.219w
  3. D12.776w.212.500w

AMA Style

References

  1. National Library of Medicine. CRISPR-Associated Protein 9. Medical Subject Headings (MeSH). 2026. Unique ID D000076987. http://id.nlm.nih.gov/mesh/2026/D000076987
  2. CRISPR-Associated Protein 9. In: Wikipedia. https://en.wikipedia.org/wiki/Cas9
  3. CRISPR-Associated Protein 9. In: Wikidata. https://www.wikidata.org/wiki/Q16965677